Anthocyanin galactosyltransferase from Aralia cordata, cDNA cloning and characterization
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چکیده
منابع مشابه
Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain.
Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycosphingolipids in mammals. We purified this enzyme over 61,000-fold to near homogeneity with a 29. 7% yield from rat brain membrane fractions. The isolation procedure included solubilization with Triton X-100, affinity chromatogr...
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In the research, genetic analysis of Aralia cordata Thunb. (Araliaceae) was conducted using randomly amplified polymorphism DNA (RAPD). 161 loci were detected with 12 RAPD primers. Percentage of Polymorphic Band (PPB) varied from 34.78% to 63.35%. All the samples were respectively collected from the eight provinces richest in Aralia cordata Thunb resources in China, including Hunan, Yunnan, Zhe...
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Profilin from common bean (Phaseolus vulgaris L.) was purified to homogeneity by poly-L-Pro affinity chromatography and gel filtration. The hypocotyl and symbiotic root nodule protein was detected as a single isoform with a 14.4-kD molecular mass and an isoelectric point of 5.3. Partial amino acid and DNA sequencing of a full-length cDNA clone confirmed its identity as profilin. An antibody gen...
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We screened for human renin inhibitory activity in wild vegetables and found the activity in methanol extract of Aralia cordata. Physico-chemical data on the isolated inhibitors were identical to those of (–)-kaur-16-en-19-oic acid (kaurenic acid, KA) and pimaradienoic acids (PDA, 8:2 mixture of pimara-8(14), 15-dien-19-oic acid: pimara-9(11) 15-dien-19-oic acid). KA and PDA also inhibited porc...
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Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a protein with 763 amino acid residues. The ded...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2004
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.21.191